Journal: Oncotarget

Article Title: Capsaicin triggers immunogenic PEL cell death, stimulates DCs and reverts PEL-induced immune suppression

doi:

Figure Lengend Snippet: Surface expression of CD14 and CD1a on immature DCs after 5 days of in-vitro differentiation in the presence or in the absence of PEL supernatant with or without Capsaicin (150 μM). One representative experiments out of three is shown. Mean fluorescence intensity is also reported.

Article Snippet: To study the role of Capsaicin in monocyte differentiation into DCs in the presence or absence of the inhibitory PEL cell supernatant, the expression of surface CD14 and CD1a was analyzed using human mAb anti-CD14 (Milteny Biotec., 130-080-701) and mAb anti-CD1a (BD Pharmingen, 555807)

Techniques: Expressing, In Vitro, Fluorescence

Journal: Cell

Article Title: Mapping Systemic Inflammation and Antibody Responses in Multisystem Inflammatory Syndrome in Children (MIS-C)

doi: 10.1016/j.cell.2020.09.034

Figure Lengend Snippet:

Article Snippet: anti-CD1c 150Nd-conjugated Clone REA694 , Miltenyi , Cat No.130-122-298; RRID: AB_2801879.

Techniques: Virus, Recombinant, Saline, Blocking Assay, Staining, Microarray, Software, Immunoprecipitation

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: HIV-1 Tat addresses dendritic cells to induce a predominant Th1-type adaptive immune response that appears prevalent in the asymptomatic stage of infection.

doi: 10.4049/jimmunol.0711406

Figure Lengend Snippet: FIGURE 6. CD1a expressing MDDC produce TNF- in response to Tat but not to cys22 Tat protein. MDDC from 12 healthy donors were cultured in the presence of native wt Tat (lot 80802) or cys22 Tat (lot 4203) (20 g/ml), or LPS (1 g/ml) for 6 and 18 h, respectively. Cells were stained (FITC) for CD1a expression, fixed, permeabilized, and then stained (PE) for TNF-. TNF--producing CD1a-positive cells were determined by flow cytometry by assessing at least 50,000 events. A, Data from a representative healthy donor, of 12 analyzed, are reported as flow cytometry dot plots. The percentages of TNF- producing CD1a cells are indicated in the panels; B, the percentages of TNF- producing CD1a cells from 12 donors are reported as mean and SEM; , p 0.01 vs medium and cys22 Tat.

Article Snippet: Cells were then washed with cold PBS containing 2% FBS and stained for 30 min at 4°C with a FITC-conjugated anti-CD1a mAb (Serotec) or an isotype-matched control mAb, fixed and permeabilized with 1 FACS lysing and permeabilizing solution (BD Biosciences Immunocytometry Systems).

Techniques: Expressing, Cell Culture, Staining, Cytometry

Journal: Clinical and Experimental Immunology

Article Title: Cerebrospinal fluid affects phenotype and functions of myeloid dendritic cells

doi: 10.1046/j.1365-2249.2002.01850.x

Figure Lengend Snippet: Influence of CSF supernatants from patients with MS (n = 34), ON (n = 10), BM (n = 5), LM (n = 5) and NIND (n = 9) on surface phenotype of donor moDC. MoDC obtained in four independent experiments were cultured for 24 h with either CSF supernatants (1/2, v/v), medium as a negative control, or rhTNF-α (100 ng/ml) as a positive control. Expression of surface markers was analysed by flow cytometry. Data are expressed as percentage MFI after culture with CSF or rhTNF-α in relation to MFI after culture with medium alone in the same experiment (background MFI). For CD1a, closed circles in the BM group indicate two cases where simultaneous up-regulation of CD14 was observed. Bars indicate medians. *P < 0·05, **P < 0·01, ***P < 0·001

Article Snippet: Cultured moDC (2–4 × 10 4 per test) were washed and incubated with fluorochrome-labelled MoAbs against CD1a, CD14, CD80, CD83, CD86 and HLA-DR (4°C, 20 min, in the dark), washed and analysed by FACScan flow cytometer and CellQuest software (both from Becton Dickinson); 10 4 events/test were acquired.

Techniques: Cell Culture, Negative Control, Positive Control, Expressing, Flow Cytometry

Journal: Journal of leukocyte biology

Article Title: Transcriptional regulation of HIV-1 LTR during antigen-dependent activation of primary T cells by dendritic cells.

doi: 10.1002/jlb.67.3.432

Figure Lengend Snippet: Fig. 7. IL-2 production and HIV replication in CD45RA1 and CD45RO1 T cell subsets activated via CD3 and CD28. (A) CD45RA1 or CD45RO1 T cells (1 3 106) were cultured with irradiated murine P815/CD80 cells (5 3 105) expressing human CD80 in the presence of anti-CD3 mAb at 5 µg/mL. IL-2 production was analyzed at day 1 as described in the legend of Figure 5. (B) CD45RA1 or CD45RO1 T cells were infected with a wild-type virus (closed circles and closed triangles, respectively) or a deletion mutant of Sp1 (open circles and open triangles, respectively). After washing, T cells were activated as in panel A. Culture medium at day 4, 7, 10, and 14 was replaced with medium containing 25 U/mL of IL-2. The level of p24 antigen in the culture supernatant was determined by ELISA.

Article Snippet: Hybridomas producing the monoclonal antibodies (mAbs) anti-CD1a (OKT6; IgG1), anti-CD3 (OKT3; IgG2a), anti-CD8 (OKT8; IgG2a), anti-CD14 (3C10; IgG2b), anti-CD21 (THB-5; IgG2a), anti-CD16 (3G8; IgG1), and anti-CD11b (OKM-1; IgG2b) were obtained from American Tissue Culture Collection (ATCC) and the IgG fraction was purified from the culture supernatant.

Techniques: Cell Culture, Irradiation, Expressing, Infection, Virus, Mutagenesis, Enzyme-linked Immunosorbent Assay